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An operational concept for long-term cinemicrography of cells in mono- and co-culture under highly controlled conditions – The SlideObserver

Paper ID Volume ID Publish Year Pages File Format Full-Text
23696 43463 2012 7 PDF Available
Title
An operational concept for long-term cinemicrography of cells in mono- and co-culture under highly controlled conditions – The SlideObserver
Abstract

Cell morphology, proliferation and motility, as well as mono- and heterotypic cell-to-cell interactions, are of increasing interest for in vitro experiments. However, tightly controlling culture conditions whilst simultaneously monitoring the same set of cells is complicated. Moreover, video-microscopy of distinct cells or areas of cells over a prolonged period of time represents a technical challenge. The SlideObserver was designed for cinemicrography of cells in co-and monoculture. The core elements of the system are the SlideReactors, miniaturised hollow fibre-based bioreactors operated in closed perfusion loops. Within the SlideReactors, cells can be cultured under adaptable conditions as well as in direct- and indirect co-culture. The independent perfusion loops enable controlled variation of parameters such as medium, pH, and oxygenation. A combined automated microscope stage and camera set-up allows for micrograph acquisition of multiple user-defined regions of interest within the bioreactor units. For proof of concept, primary cells (HUVEC, human hepatocytes) and cell lines (HuH7, THP-1) were cultured under stable and varying culture conditions, as well as in mono- and co-culture. The operational system enabled non-stop imaging and automated control of process parameters as well as elective manipulation of either reactor. As opposed to non-perfused culture systems or comparable devices for cinemicrographic analysis, the SlideObserver allows simultaneous morphological monitoring of an entire culture of cells in multiple bioreactors.

► Parallel time-lapse recording of different sets of cells in culture is challenging. ► We developed the SlideObserver for long-term culture and video-microscopy. ► The SlideObserver allows exact control of culture parameter. ► Seamless cinemicrography of primary cells for up to three weeks was possible. ► Long-term cinemicrography of cultured cells deliver insights into cellular behaviour.

Keywords
Time lapse; Cinemicrography; Hepatocytes; Coculture; Bioreactor
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An operational concept for long-term cinemicrography of cells in mono- and co-culture under highly controlled conditions – The SlideObserver
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 159, Issues 1–2, 31 May 2012, Pages 83–89
Authors
, , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us