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Production of flavor esters catalyzed by CALB-displaying Pichia pastoris whole-cells in a batch reactor

Paper ID Volume ID Publish Year Pages File Format Full-Text
23699 43463 2012 7 PDF Available
Title
Production of flavor esters catalyzed by CALB-displaying Pichia pastoris whole-cells in a batch reactor
Abstract

Candida antarctica lipase B (CALB) has been employed as an efficient catalyst in the preparation of many flavor esters. A CALB-displaying yeast whole-cell biocatalyst could be an attractive alternative to commercial immobilized CALB because of its low-cost preparation and high enzymatic activity. We investigated the potential application of CALB-displaying Pichia pastoris cells for the production of flavor esters. The optimal conditions for flavor esters synthesis by this biocatalyst were determined in 50-ml shake flasks. Under optimized conditions, the synthesis of 12 kinds flavor esters were scaled up in a 5-l batch stirred reactor. Among these, the mole conversions of 10 exceeded 95% after reactions for 4 h. In addition, this biocatalyst showed good tolerance for high substrates concentration and excellent operational stability. Repeated use of the cells in 10 batches resulted in an activity loss of less than 10%. Thus, CALB-displaying P. pastoris whole cells are robust biocatalysts with potential commercial application in the large-scale production of flavor esters in non-aqueous media.

► CALB-displaying P. pastoris exhibited high synthetic activity as a whole-cell biocatalysts. ► It showed good tolerance for temperature and high substrate concentrations. ► 12 kinds of flavor esters were synthesized very efficiently in a 5-l batch reactor. ► This biocatalyst is promising for potential use for the commercial production.

Keywords
Yeast surface display; Candida antarctica lipase B; Flavor esters; Enzymatic synthesis; Batch reactor production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 159, Issues 1–2, 31 May 2012, Pages 108–114
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us