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Arabidopsis-derived shrimp viral-binding protein, PmRab7 can protect white spot syndrome virus infection in shrimp

Paper ID Volume ID Publish Year Pages File Format Full-Text
23708 43465 2012 8 PDF Available
Title
Arabidopsis-derived shrimp viral-binding protein, PmRab7 can protect white spot syndrome virus infection in shrimp
Abstract

White spot syndrome virus is currently the leading cause of production losses in the shrimp industry. Penaeus monodon Rab7 protein has been recognized as a viral-binding protein with an efficient protective effect against white spot syndrome infection. Plant-derived recombinant PmRab7 might serve as an alternative source for in-feed vaccination, considering the remarkable abilities of plant expression systems. PmRab7 was introduced into the Arabidopsis thaliana T87 genome. Arabidopsis-derived recombinant PmRab7 showed high binding activity against white spot syndrome virus and a viral envelope, VP28. The growth profile of Arabidopsis suspension culture expressing PmRab7 (ECR21# 35) resembled that of its counterpart. PmRab7 expression in ECR21# 35 reached its maximum level at 5 mg g−1 dry weight in 12 days, which was higher than those previously reported in Escherichia coli and in Pichia. Co-injection of white spot syndrome virus and Arabidopsis crude extract containing PmRab7 in Litopenaeus vannamei showed an 87% increase in shrimp survival rate at 5 day after injection. In this study, we propose an alternative PmRab7 source with higher production yield, and cheaper culture media costs, that might serve the industry's need for an in-feed supplement against white spot syndrome infection.

► Arabidopsis-derived rPmRab7 is an alternative source of WSSV protective protein. ► AtrPmRab7 production (5 mg g−1 DW) was higher than previously reported in Pichia. ► Co-injection of AtrPmRab7 and WSSV increased Peneaus vannamei survival rate by 87%. ► AtrPmRab7 showed feasibility to serve industrial needs for shrimp feed supplement.

Keywords
rPmRab7, recombinant Penaeus monodon Rab7 protein; WSSV, White spot syndrome virus; AtrPmRab7, Arabidopsis-derived rPmRab7 protein; rVP28, recombinant VP28 proteinPlant-derived protective protein; Rab7; White spot syndrome virus; Penaeus monodon; Arabidop
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Arabidopsis-derived shrimp viral-binding protein, PmRab7 can protect white spot syndrome virus infection in shrimp
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 161, Issue 1, 15 September 2012, Pages 60–67
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us