fulltext.study @t Gmail

Heterologous expression of Aspen PTM3, a MADS box gene in cotton

Paper ID Volume ID Publish Year Pages File Format Full-Text
23712 43467 2011 7 PDF Available
Title
Heterologous expression of Aspen PTM3, a MADS box gene in cotton
Abstract

The PTM3 gene of Aspen was ectopically expressed in cotton to explore the opportunity to introduce desirable agronomic traits with the potential to improve yield and modify the duration of the parent cotton variety. Sixty-seven transgenic cotton lines expressing Aspen PTM3 (MADS box) gene were developed. The transgenic cotton lines expressing PTM3 gene showed earliness of 4–15 days variations in flowering and maturity. The transgenic lines were confirmed by kanamycin leaf paint assay, GUS assay and PCR. Among 67 transgenic lines, the event-10 showed profuse branching, event-24 showed abnormal growth and the remaining events exhibited single erect phenotype. In addition, the event-24 produced no flower and this might be due to the positional effect of PTM3 gene integration. Southern blot analysis performed for event-10, 24 and 48 showed distinct single copy integrations of PTM3 gene cassette. GUS assay performed using various plant parts of event-10 showed constitutive expression of the transgene. In view of cotton breeding, among all the events, the event-10 was found to be phenotypically significant with earliness of 12 days in flowering and 15 days in maturity and yield enhancement of 27%. In addition, the event-10 showed no square dropping and allowed the plants to bear more number of bolls. Based on these results, event-10 was chosen to carryout the inheritance study of expressed characters in the progeny.

Keywords
BCIP, 5-bromo, 4-chloro, 3-indolyl phosphate; 2,4-D, 2,4-dichlorophenoxy-acetic acid; PTM3, Populus tremuloides MADS-box3; NBT, nitro blue tetrozolium; NPTII, neomycin phosphotransferase II; T0, transgenic plant developed from tissue cultureAgrobacterium-
First Page Preview
Heterologous expression of Aspen PTM3, a MADS box gene in cotton
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 155, Issue 2, 10 September 2011, Pages 140–146
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us