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Highly regioselective glucosylation of 2′-deoxynucleosides by using the crude β-glycosidase from bovine liver

Paper ID Volume ID Publish Year Pages File Format Full-Text
23720 43467 2011 6 PDF Available
Title
Highly regioselective glucosylation of 2′-deoxynucleosides by using the crude β-glycosidase from bovine liver
Abstract

An enzymatic regioselective approach for the glucosylation of a series of 2′-deoxynucleosides was described by using the crude β-glycosidase from bovine liver that is less expensive and can be simply prepared in a standard organic laboratory. With the glucosylation of 2′-deoxyuridine as a model reaction, the effects of several key factors on the enzymatic reaction were examined. The optimum enzyme dosage, buffer pH and temperature were 0.05 U/ml, 9.5 and 42 °C, respectively. The presence of alkali β-glycosidase as the main active component in the crude enzyme extract might account for the high glucosylation activity at pH 9.5. In addition, the desired 5′-O-glucosylated derivatives of 2′-deoxynucleosides were synthesized with the yields of 22–72% and exclusive 5′-regioselectivities (>99%).

Keywords
Bovine liver; Disaccharide nucleosides; β-Glycosidase; Glucosylation; Regioselectivity
First Page Preview
Highly regioselective glucosylation of 2′-deoxynucleosides by using the crude β-glycosidase from bovine liver
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 155, Issue 2, 10 September 2011, Pages 203–208
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering