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Reducing agents improve enzymatic hydrolysis of cellulosic substrates in the presence of pretreatment liquid

Paper ID Volume ID Publish Year Pages File Format Full-Text
23726 43467 2011 7 PDF Available
Title
Reducing agents improve enzymatic hydrolysis of cellulosic substrates in the presence of pretreatment liquid
Abstract

Enzymatic hydrolysis of pretreated lignocellulosic substrates has emerged as an interesting option to produce sugars that can be converted to liquid biofuels and other commodities using microbial biocatalysts. Lignocellulosic substrates are pretreated to make them more accessible to cellulolytic enzymes, but the pretreatment liquid partially inhibits subsequent enzymatic hydrolysis. The presence of pretreatment liquid from Norway spruce resulted in a 63% decrease in the enzymatic saccharification of Avicel compared to when the reaction was performed in a buffered aqueous solution. The addition of 15 mM of a reducing agent (hydrogen sulfite, dithionite, or dithiothreitol) to reaction mixtures with the pretreatment liquid resulted in up to 54% improvement of the saccharification efficiency. When the reducing agents were added to reaction mixtures without pretreatment liquid, there was a 13–39% decrease in saccharification efficiency. In the presence of pretreatment liquid, the addition of 15 mM dithionite to Avicel, α-cellulose or filter cake of pretreated spruce wood resulted in improvements between 25 and 33%. Positive effects (6–17%) of reducing agents were also observed in experiments with carboxymethyl cellulose and 2-hydroxyethyl cellulose. The approach to add reducing agents appears useful for facilitating the utilization of enzymes to convert cellulosic substrates in industrial processes.

► Pretreatment liquid inhibited enzymatic hydrolysis of cellulose. ► Addition of reducing agents to the pretreatment liquid made enzymatic hydrolysis of cellulose more efficient. ► Without pretreatment liquid, inclusion of reducing agents had a negative effect on the enzymatic hydrolysis. ► The reducing agents investigated includedhydrogen sulfite, dithionite, and dithiothreitol.

Keywords
Saccharification; Enzymatic hydrolysis; Cellulase; Cellulose; Reducing agents
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Reducing agents improve enzymatic hydrolysis of cellulosic substrates in the presence of pretreatment liquid
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 155, Issue 2, 10 September 2011, Pages 244–250
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us