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Cell selective targeting of a simian virus 40 virus-like particle conjugated to epidermal growth factor

Paper ID Volume ID Publish Year Pages File Format Full-Text
23727 43467 2011 6 PDF Available
Title
Cell selective targeting of a simian virus 40 virus-like particle conjugated to epidermal growth factor
Abstract

Simian virus 40 (SV40) virus-like particles (VLPs) are efficient nanocarriers for gene delivery. VLPs conjugated to human epidermal growth factor (hEGF) were prepared and the cell selectivity of the VLP was examined using human epithelial carcinoma A431 cells, which overexpress the EGF receptor. The endocytic efficiency was determined by the level of Gaussia luciferase activity from the encapsulated protein in hEGF-conjugated VLPs. EGF receptor-mediated endocytosis of hEGF-conjugated VLPs was significantly increased and was confirmed by fluorescence imaging using mCherry encapsulated in hEGF-conjugated VLPs. These results suggest that VLPs of SV40 conjugated to a specific ligand could be used for cell selective gene delivery.

Keywords
SV40, simian virus 40; VLP, virus-like particle; hEGF, human epidermal growth factor; GLase, Gaussia luciferase; VLPN138C, N138C VP1 VLP; GV, GLase encapsulated in VLPN138C; VE, hEGF conjugated VLPN138C; GVE, GLase encapsulated in VLPN138C conjugating hEG
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Cell selective targeting of a simian virus 40 virus-like particle conjugated to epidermal growth factor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 155, Issue 2, 10 September 2011, Pages 251–256
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us