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Gelatin enhances 2-keto-l-gulonic acid production based on Ketogulonigenium vulgare genome annotation

Paper ID Volume ID Publish Year Pages File Format Full-Text
23734 43468 2011 6 PDF Available
Title
Gelatin enhances 2-keto-l-gulonic acid production based on Ketogulonigenium vulgare genome annotation
Abstract

In the two-step fermentative production of vitamin C, its precursor 2-keto-l-gulonic acid (2-KLG) was synthesized by Ketogulonicigenium vulgare through co-culture with Bacillus megaterium. The reconstruction of the amino acid metabolic pathway through completed genome sequence annotation demonstrated that K. vulgare was deficient in one or more key enzymes in the de novo biosynthesis pathways of eight different amino acids (l-histidine, l-glycine, l-lysine, l-proline, l-threonine, l-methionine, l-leucine, and l-isoleucine). Among them, l-glycine, l-proline, l-threonine, and l-isoleucine play vital roles in K. vulgare growth and 2-KLG production. The addition of those amino acids increased the 2-KLG productivity by 20.4%, 17.2%, 17.2%, and 11.8%, respectively. Furthermore, food grade gelatin was developed as a substitute for the amino acids to increase the cell concentration, 2-KLG productivity, and l-sorbose consumption rate by 10.2%, 23.4%, and 20.9%, respectively. As a result, the fermentation period decreased to 43 h in a 7-L fermentor.

► The amino acid metabolic pathway in Ketogulonigenium vulgare was elucidated through genome annotation. ► l-Glycine, l-proline, l-threonine, and l-isoleucine play key role on the K. vulgare growth and 2-KLG production. ► Food grade gelatin was developed to increase 2-keto-l-gulonic acid production.

Keywords
Gelatin; Genome annotation; Ketogulonicigenium vulgare; 2-Keto-l-gulonic acid
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Gelatin enhances 2-keto-l-gulonic acid production based on Ketogulonigenium vulgare genome annotation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 156, Issue 3, 10 December 2011, Pages 182–187
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us