fulltext.study @t Gmail

Rapid selection of single cells with high antibody production rates by microwell array

Paper ID Volume ID Publish Year Pages File Format Full-Text
23736 43468 2011 6 PDF Available
Title
Rapid selection of single cells with high antibody production rates by microwell array
Abstract

Selection of single cells capable of producing target proteins at high rates is crucial for the development of protein manufacturing processes. Here, we present the rapid selection of single cells producing immunoglobulin antibodies at high specific rates by microwell array and microengraving. Chinese hamster ovary (CHO) cells secreting chimeric antibodies were deposited in a microwell array in a manner such that each microwell contained a single cell. Secreted antibodies in the microwells were transferred onto a glass slide by microengraving, followed by interrogation using fluorescence-based immunoassay. Single cells displaying high signal intensities were selected, retrieved, and clonally expanded to assess their specific antibody production rates. Three successive rounds of the process resulted in the selection of single cells showing significantly increased antibody production rates. The present approach can be applied to the selection of single cells for producing other therapeutic proteins in a high-throughput manner.

► We present an efficient method to select single CHO cells producing human antibody at high producing rates using microwell array and microengraving. ► A successful selection of single cells with high production rates was achieved by three rounds of selection process. ► The present approach can be applied to the selection of single mammalian cell lines for the producing other therapeutic proteins in a high-throughput manner.

Keywords
Single cell; Antibody; Microwells; Microengraving; CHO cell
First Page Preview
Rapid selection of single cells with high antibody production rates by microwell array
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 156, Issue 3, 10 December 2011, Pages 197–202
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us