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Using Vaccinia's innate ability to introduce DNA into mammalian cells for production of recombinant proteins

Paper ID Volume ID Publish Year Pages File Format Full-Text
23738 43468 2011 3 PDF Available
Title
Using Vaccinia's innate ability to introduce DNA into mammalian cells for production of recombinant proteins
Abstract

Production of recombinant protein in mammalian cells is time-consuming, labor-intensive and costly. While seeking to overcome these limitations, we discovered that Vaccinia virus has the innate ability to transfer exogenous plasmid DNA into mammalian cells during the infection process. Parameters influencing the efficiency of this event were characterized and a quick, simple and inexpensive way to produce eukaryotic proteins was established.

► We discover that Vaccinia virus has the innate ability to transfer exogenous DNA into mammalian cells during infection. ► We optimize the conditions of this viral transfer of DNA and develop a new protocol to produce proteins in mammalian cells. ► The newly developed methods have several advantages and are equal to or superior in performance when compared to standard methods.

Keywords
Recombinant; Vaccinia; T7; Transfection; Infection; MVA
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Using Vaccinia's innate ability to introduce DNA into mammalian cells for production of recombinant proteins
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 156, Issue 3, 10 December 2011, Pages 211–213
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us