fulltext.study @t Gmail

Identification and functional characterization of phenylglycine biosynthetic genes involved in pristinamycin biosynthesis in Streptomyces pristinaespiralis

Paper ID Volume ID Publish Year Pages File Format Full-Text
23750 43470 2011 5 PDF Available
Title
Identification and functional characterization of phenylglycine biosynthetic genes involved in pristinamycin biosynthesis in Streptomyces pristinaespiralis
Abstract

Pristinamycin I (PI), a streptogramin type B antibiotic produced by Streptomyces pristinaespiralis, contains the aproteinogenic amino acid l-phenylglycine. Recent sequence analysis led to the identification of a set of putative phenylglycine biosynthetic genes. Successive inactivation of the individual genes resulted in a loss of PI production. Production was restored by supplementation with externally added l-phenylglycine, which demonstrates that these genes are involved in phenylglycine biosynthesis and thus probably disclosing the last essential pristinamycin biosynthetic genes. Finally, a putative pathway for phenylglycine synthesis is proposed.

Keywords
Dpg, 3,5-dihydroxyphenylglycine; Hpg, 4-hydroxyphenylglycine; HPLC, high performance liquid chromatography; HPLC-DAD, HPLC-diode array detection; MRSA, methicillin-resistant Staphylococcus aureus; NRPS, nonribosomal peptide synthetase; ORF, open reading f
First Page Preview
Identification and functional characterization of phenylglycine biosynthetic genes involved in pristinamycin biosynthesis in Streptomyces pristinaespiralis
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 155, Issue 1, 20 August 2011, Pages 63–67
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering