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BpiB05, a novel metagenome-derived hydrolase acting on N-acylhomoserine lactones

Paper ID Volume ID Publish Year Pages File Format Full-Text
23753 43470 2011 9 PDF Available
Title
BpiB05, a novel metagenome-derived hydrolase acting on N-acylhomoserine lactones
Abstract

The N-acyl-homoserine lactones (N-AHLs) play an important role in bacterial cell–cell signaling. Up to date, however, only a few different experimentally proven classes of N-AHL ring-cleaving enzymes are known. Here we report on the isolation and biochemical characterization of a novel hydrolase derived from the soil metagenome and acting on N-AHLs. The identified protein designated BpiB05 is weakly similar to hypothetical proteins from Bacteroides fragilis, the draft genomes of two Burkholderia species as well as a marine metagenomic ORF but is otherwise not similar to any known protein. BpiB05 was overexpressed in Escherichia coli as a 10× His-tagged fusion protein. The recombinant protein revealed a molecular weight of about 70 kDa and was tested for its quorum quenching (QQ) activities using a lacZ-bioassay. Additional HPLC–MS analyses confirmed the lactonolytic activity of the purified protein in the presence of Ca2+. Further tests suggested that BpiB05 strongly reduces motility in Pseudomonas aeruginosa, pyocyanin synthesis and biofilm formation in this microbe. Because BpiB05 is not distantly related to any of the currently known hydrolases it forms probably a novel group within the growing number of proteins acting on N-AHLs.

Keywords
Homoserine lactone; Hydrolase; Metagenomic; Quorum sensing; Pseudomonas aeruginosa
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BpiB05, a novel metagenome-derived hydrolase acting on N-acylhomoserine lactones
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 155, Issue 1, 20 August 2011, Pages 86–94
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us