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Regulation of the malic enzyme gene malE by the transcriptional regulator MalR in Corynebacterium glutamicum

Paper ID Volume ID Publish Year Pages File Format Full-Text
23782 43472 2012 12 PDF Available
Title
Regulation of the malic enzyme gene malE by the transcriptional regulator MalR in Corynebacterium glutamicum
Abstract

Corynebacterium glutamicum is a Gram-positive nonpathogenic bacterium that is used for the biotechnological production of amino acids. Here, we investigated the transcriptional control of the malE gene encoding malic enzyme (MalE) in C. glutamicum ATCC 13032, which is known to involve the nitrogen regulator AmtR. Gel shift experiments using purified regulators RamA and RamB revealed binding of these regulators to the malE promoter. In DNA-affinity purification experiments a hitherto uncharacterized transcriptional regulator belonging to the MarR family was found to bind to malE promoter DNA and was designated as MalR. C. glutamicum cells overexpressing malR showed reduced MalE activities in LB medium or in minimal media with acetate, glucose, pyruvate or citrate. Deletion of malR positively affected MalE activities during growth in LB medium and minimal media with pyruvate, glucose or the TCA cycle dicarboxylates l-malate, succinate and fumarate. Transcriptional fusion analysis revealed elevated malE promoter activity in the malR deletion mutant during growth in pyruvate minimal medium suggesting that MalR acts as a repressor of malE. Purified MalR bound malE promoter DNA in gel shift experiments. Two MalR binding sites were identified in the malE promoter by mutational analysis. Thus, MalR contributes to the complex transcriptional control of malE which also involves RamA, RamB and AmtR.

► We characterized MalR as transcriptional regulator of malE in Corynebacterium glutamicum. ► MalR binds to the consensus binding sequence TTTc/tt/cGTt/c/ac/g. ► Transcriptional control of malE is complex and involves RamA, RamB, AmtR and MalR.

Keywords
Corynebacterium glutamicum; Transcriptional regulation; Malic enzyme; MalR; MalE
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Regulation of the malic enzyme gene malE by the transcriptional regulator MalR in Corynebacterium glutamicum
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 159, Issue 3, 15 June 2012, Pages 204–215
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us