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Activity and diversity of haloalkaliphilic methanogens in Central Asian soda lakes

Paper ID Volume ID Publish Year Pages File Format Full-Text
23798 43473 2012 7 PDF Available
Title
Activity and diversity of haloalkaliphilic methanogens in Central Asian soda lakes
Abstract

Methanogens are of biotechnological interest because of their importance in biogas production. Here we investigate the suitability of sediments from Central Asian soda lakes as inoculum for high pH methane-producing bioreactors. Methane production in these sediments was modest (up to 2.5 μmol mL sediment), with methanol and hydrogen as the preferred substrates. The responsible methanogenic community was characterized based on mcrA gene sequences. McrA gene sequences so far specific to these habitats indicated the presence of two clusters within the orders Methanobacteriales and Methanomicrobiales, one apparently including representatives of the genus Methanocalculus and another distantly related to the genus Methanobacterium.

► Soda lake sediments as promising inoculum for high pH methane producing bioreactors. ► Methane production in sediments with substrates methanol and hydrogen at pH 9.5–10. ► Novel mcrA gene sequences amplified from soda lake sediment DNA extracts. ► Results indicate presence of specialized haloalkaliphilic methanogenic populations.

Keywords
Methanogenesis; Soda lakes; McrA gene; Biogas
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Activity and diversity of haloalkaliphilic methanogens in Central Asian soda lakes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 161, Issue 2, 15 October 2012, Pages 167–173
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us