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Metabolic engineering of Pseudomonas fluorescens for the production of vanillin from ferulic acid

Paper ID Volume ID Publish Year Pages File Format Full-Text
23811 43474 2011 8 PDF Available
Title
Metabolic engineering of Pseudomonas fluorescens for the production of vanillin from ferulic acid
Abstract

Vanillin is one of the most important flavors in the food industry and there is great interest in its production through biotechnological processes starting from natural substrates such as ferulic acid. Among bacteria, recombinant Escherichia coli strains are the most efficient vanillin producers, whereas Pseudomonas spp. strains, although possessing a broader metabolic versatility, rapidly metabolize various phenolic compounds including vanillin. In order to develop a robust Pseudomonas strain that can produce vanillin in high yields and at high productivity, the vanillin dehydrogenase (vdh)-encoding gene of Pseudomonas fluorescens BF13 strain was inactivated via targeted mutagenesis. The results demonstrated that engineered derivatives of strain BF13 accumulate vanillin if inactivation of vdh is associated with concurrent expression of structural genes for feruloyl-CoA synthetase (fcs) and hydratase/aldolase (ech) from a low-copy plasmid. The conversion of ferulic acid to vanillin was enhanced by optimization of growth conditions, growth phase and parameters of the bioconversion process. The developed strain produced up to 8.41 mM vanillin, which is the highest final titer of vanillin produced by a Pseudomonas strain to date and opens new perspectives in the use of bacterial biocatalysts for biotechnological production of vanillin from agro-industrial wastes which contain ferulic acid.

► We developed a new Pseudomonas-based platform for the biosynthesis of vanillin from ferulic acid. ► Effective strategy applicable to other bioprocesses which involve conversion of phenylpropenoids into CoA-derivatives. ► The first study on high-rate vanillin production by Pseudomonas strains. ► Vanillin production using resting cells of this novel strain is not accompanied by the formation of toxic by-products.

Keywords
Vanillin production; Pseudomonas; Ferulic acid; Bioconversion; Whole-cell biocatalyst; Metabolic engineering
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Metabolic engineering of Pseudomonas fluorescens for the production of vanillin from ferulic acid
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 156, Issue 4, 20 December 2011, Pages 309–316
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us