fulltext.study @t Gmail

Modulating the synthetase activity of penicillin G acylase in organic media by addition of N-methylimidazole: Using vinyl acetate as activated acyl donor

Paper ID Volume ID Publish Year Pages File Format Full-Text
23826 43476 2011 5 PDF Available
Title
Modulating the synthetase activity of penicillin G acylase in organic media by addition of N-methylimidazole: Using vinyl acetate as activated acyl donor
Abstract

This paper reported the modulation of enzyme activity by organic small molecule. The esterification activity of Penicillin G acylase (PGA) was improved more than 70-fold by the addition of 10% N-methylimidazole. Some control experiments have been designed to demonstrate the catalytic specificity of PGA. The structure and the amount of additive were optimized to improve the product yield. The influence of N-methylimidazole on the PGA conformation was investigated by FTIR and autodock simulation. Seven substrates were used to evaluate the effect of structure on the PGA-catalyzed transesterification. A series of products were successfully synthesized with the yield ranged from 56% to 84% and PGA showed specific recognition on the substrate with phenyl group in the presence of 10% N-methylimidazole.

Keywords
Acylase; Enzymatic synthesis; Esterification; N-methylimidazole
First Page Preview
Modulating the synthetase activity of penicillin G acylase in organic media by addition of N-methylimidazole: Using vinyl acetate as activated acyl donor
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 153, Issues 3–4, 20 May 2011, Pages 111–115
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us