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Biosynthesis and characterization of CdS quantum dots in genetically engineered Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
23828 43476 2011 8 PDF Available
Title
Biosynthesis and characterization of CdS quantum dots in genetically engineered Escherichia coli
Abstract

Quantum dots (QDs) were prepared in genetically engineered Escherichia coli (E. coli) through the introduction of foreign genes encoding a CdS binding peptide. The CdS QDs were successfully separated from the bacteria through two methods, lysis and freezing–thawing of cells, and purified with an anion-exchange resin. High-resolution transmission electron microscopy, X-ray diffraction, luminescence spectroscopy, and energy dispersive X-ray spectroscopy were applied to characterize the as-prepared CdS QDs. The effects of reactant concentrations, bacteria incubation times, and reaction times on QD growth were systematically investigated. Our work demonstrates that genetically engineered bacteria can be used to synthesize QDs. The biologically synthesized QDs are expected to be more biocompatible probes in bio-labeling and imaging.

Keywords
Biosynthesis; Genetic engineering; Escherichia coli; CdS
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Biosynthesis and characterization of CdS quantum dots in genetically engineered Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 153, Issues 3–4, 20 May 2011, Pages 125–132
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
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