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Calorimetric control of the specific growth rate during fed-batch cultures of Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
23861 43478 2012 7 PDF Available
Title
Calorimetric control of the specific growth rate during fed-batch cultures of Saccharomyces cerevisiae
Abstract

The specific growth rate of a Saccharomyces cerevisiae strain with glucose as limiting C-source was estimated from the measured heat flow produced by the cells. For the cultivation a standard 30 l laboratory bioreactor was used, which was extended in such a way that heat balancing is possible. The feed rate was adjusted by a feedforward/feedback controller such that the specific growth rate was kept on the desired set-point value. On the basis of experimental investigations it was demonstrated that the specific growth rate can be controlled at a given set point value below the critical value to prevent the production of growth-inhibitory ethanol due to the Crabtree effect. With this control strategy high biomass concentrations of more than 110 g l−1 can be obtained.

► A standard 30 l laboratory bioreactor was extended in such a way that heat balancing is possible. ► The specific growth rate of a Saccharomyces cerevisiae strain can be estimated from the measured heat flow produced by the cells. ► The specific growth rate can be controlled at a given set point value below the critical value to prevent the production of growth-inhibitory ethanol due to the Crabtree effect.

Keywords
Calorimetry; Heat flow; Saccharomyces cerevisiae; Specific growth rate; Fed-batch; Control
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Calorimetric control of the specific growth rate during fed-batch cultures of Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 160, Issues 3–4, 31 August 2012, Pages 195–201
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us