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Temperature-responsive glass coverslips with an ultrathin poly(N-isopropylacrylamide) layer

Paper ID Volume ID Publish Year Pages File Format Full-Text
2387 109 2009 7 PDF Available
Title
Temperature-responsive glass coverslips with an ultrathin poly(N-isopropylacrylamide) layer
Abstract

A temperature-responsive cross-linked polymer gel was covalently grafted onto glass coverslips by electron beam irradiation. The grafted thickness and amount of polymer as well as the surface wettability increased with the initial monomer concentration. When the monomer concentration was 5 wt.%, the grafted polymer density was 0.84 μg cm−2, and cells adhered and spread on the surface at 37 °C, but detached at 20 °C. In contrast, when the monomer concentration was 35 wt.%, the polymer density was 1.28 μg cm−2, and the surfaces were cell repellent even at 37 °C. These results show a remarkable contrast to those obtained from temperature-responsive polymer-grafted tissue culture polystyrene dishes, since various types of cells showed temperature-dependent cell adhesion/detachment when the grafted density was around 2 μg cm−2 on these surfaces. We discuss the possible molecular mechanisms underlying this discrepancy.

Keywords
Poly(N-isopropylacrylamide) (PIPAAm); Electron beam; Cell adhesion; Cell detachment
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 5, Issue 1, January 2009, Pages 470–476
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us