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Generalized semi-refolding methods for purification of the functional death domain superfamily

Paper ID Volume ID Publish Year Pages File Format Full-Text
23895 43481 2011 8 PDF Available
Title
Generalized semi-refolding methods for purification of the functional death domain superfamily
Abstract

The death domain (DD) superfamily comprising the death domain (DD) subfamily, the death effector domain (DED) subfamily, the caspase recruitment domain (CARD) subfamily and the pyrin domains (PYD) subfamily is one of the largest classes of protein interaction modules and plays a pivotal role in the apoptosis, inflammation, and immune cell signaling pathways. Despite the biological importance of the death domain superfamily, structural and in vitro biochemical studies have been limited because these domains are prone to aggregate under physiological conditions. Here, we describe a generalized method, termed semi-refolding, that is particularly applicable for purification of the functional death domain superfamily. The recombinant proteins Caspase-1 CARD, AIM2 PYD, NALP3 PYD, and RIP1 DD from inclusion bodies were successfully purified using this method.

Keywords
Apoptosis; Inflammation; Death domain superfamily; Purification; Refolding
First Page Preview
Generalized semi-refolding methods for purification of the functional death domain superfamily
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 151, Issue 4, 20 February 2011, Pages 335–342
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering