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Determination of phenanthrene bioavailability by using a self-dying reporter bacterium: Test with model solids and soil

Paper ID Volume ID Publish Year Pages File Format Full-Text
23903 43482 2012 6 PDF Available
Title
Determination of phenanthrene bioavailability by using a self-dying reporter bacterium: Test with model solids and soil
Abstract

The present study was conducted to investigate the performance and feasibility of a self-dying reporter bacterium to visualize and quantify phenanthrene bioavailability in soil. The self-dying reporter bacterium was designed to die on the initiation of phenanthrene biodegradation. The viability of the reporter bacterium was determined by a fluorescence live/dead cell staining method and visualized by confocal laser scanning microscopic observation. Phenanthrene was spiked into four types of model solids and a sandy loam. The bioavailability of phenanthrene to the reporter bacterium was remarkably declined with the hydrophobicity of the model solids: essentially no phenanthrene was biodegraded in the presence of 9-nm pores and about 35.8% of initial phenanthrene was biodegraded without pores. Decrease in bioavailability was not evident in the nonporous hydrophilic bead, but a small decrease was observed in the porous hydrophilic bead at 1000 mg/kg of phenanthrene. The fluorescence intensity was commensurate with the extent of phenanthrene biodegradation by the reporter bacterium at the concentration range from 50 to 500 mg/kg. Such a quantitative relationship was also confirmed with a sandy loam spiked up to 1000 mg/kg of phenanthrene. This reporter bacterium may be a useful means to determine phenanthrene bioavailability in soil.

► The performance of a self-dying reporter bacterium to determine phenanthrene bioavailability has been tested with model solids and soil. ► The bioavailability of phenanthrene was able to be quantitatively determined by the self-dying reporter bacterium. ► In soil, the quantitative relationship was more precise in the self-dying reporter bacterium than a conventional bioreporter strain.

Keywords
Phenanthrene; Model solid; Soil; Bioavailability; Visualization; Self-dying reporter bacterium
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Determination of phenanthrene bioavailability by using a self-dying reporter bacterium: Test with model solids and soil
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 157, Issue 4, 20 February 2012, Pages 454–459
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us