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Sustained H2 production in a Chlamydomonas reinhardtii D1 protein mutant

Paper ID Volume ID Publish Year Pages File Format Full-Text
23922 43482 2012 7 PDF Available
Title
Sustained H2 production in a Chlamydomonas reinhardtii D1 protein mutant
Abstract

In the present investigation, a detailed biochemical analysis of the high H2 producer D1 protein mutant strain L159I-N230Y of Chlamydomonas reinhardtii, carrying a double amino acid substitution, was made. The leucine residue L159 was replaced by isoleucine, and the N230 asparagine was replaced by tyrosine. The performance of this strain was compared to that of the cc124 strain. The mutant showed a sustained capacity to donate electrons by means of direct biophotolysis for H2 production, as demonstrated by the higher efficiency of utilization of the hydrogenase enzyme when carried out under anaerobic conditions. The latter property was maintained also under sulfur deprivation. Furthermore, when compared to the cc124, the mutant showed a higher amount of D1 protein content, a higher carbohydrate storage capacity and a sustained PSII direct contribution to the H2 production during sulfur deprivation. The addition of DCMU to the cells showed that as much as 7.0 mL H2 liter of culture h−1 were produced by means of direct biophotolysis. The maximum apparent light-to-hydrogen conversion efficiency expressed on PAR (photosynthetically active radiation) reached 3.22%, while PSII efficiency to perform direct biophotolysis was calculated to be 2.03%. These values are significantly higher than what has been reported in the literature.

Keywords
H2 production; Biophotolysis; D1 protein; Chlamydomonas reinhardtii
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Sustained H2 production in a Chlamydomonas reinhardtii D1 protein mutant
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 157, Issue 4, 20 February 2012, Pages 613–619
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us