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Defining a process operating window for the synthesis of 5-methyluridine by transglycosylation of guanosine and thymine

Paper ID Volume ID Publish Year Pages File Format Full-Text
23939 43483 2011 6 PDF Available
Title
Defining a process operating window for the synthesis of 5-methyluridine by transglycosylation of guanosine and thymine
Abstract

This paper describes a high yielding coupled enzymatic reaction using Bacillus halodurans purine nucleoside phosphorylase (PNP) and E. coli uridine phosphorylase (UP) for synthesis of 5-methyluridine (5-MU) by transglycosylation. Key parameters such as reaction temperature, pH, reactant loading, reactor configuration and enzyme loading were investigated. A guanosine conversion of 95% and a 5-MU yield of 85% were achieved at 1 l scale, with a productivity of 10 g l−1 h−1.

Keywords
Biocatalysis; 5-Methyluridine; Nucleoside phosphorylase; Cascade reaction; Antiretroviral
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Defining a process operating window for the synthesis of 5-methyluridine by transglycosylation of guanosine and thymine
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 151, Issue 1, 10 January 2011, Pages 108–113
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
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