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The pstSCAB operon for phosphate uptake is regulated by the global regulator GlxR in Corynebacterium glutamicum

Paper ID Volume ID Publish Year Pages File Format Full-Text
23952 43484 2011 7 PDF Available
Title
The pstSCAB operon for phosphate uptake is regulated by the global regulator GlxR in Corynebacterium glutamicum
Abstract

The pstSCAB operon of Corynebacterium glutamicum, which encodes a high affinity transport system for uptake of the phosphorus source inorganic phosphate, is induced upon phosphate starvation involving activation by the two-component regulatory system PhoS–PhoR. Partial phosphate starvation induction of the pstSCAB operon in a ΔphoRS mutant indicated the involvement of (an) additional transcriptional regulator(s). Here, GlxR, a global cAMP-dependent transcriptional regulator, was shown to bind to the pstS promoter −133 bp to −117 bp upstream of the transcriptional start site as shown by gel shift and mutation experiments. Transcriptional fusion analysis revealed that GlxR activates the pstSCAB operon under phosphate limiting conditions in a carbon source dependent manner. Commensurate with these findings, overexpression of glxR was shown to stimulate growth under phosphate limiting conditions with glucose, but not with acetate, as carbon source. Thus, in C. glutamicum pstSCAB expression is regulated in response to the availability of phosphorus and carbon sources.

Keywords
Corynebacterium glutamicum; Phosphate starvation; pstSCAB; Transcriptional regulation; GlxR
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The pstSCAB operon for phosphate uptake is regulated by the global regulator GlxR in Corynebacterium glutamicum
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 154, Issues 2–3, 10 July 2011, Pages 149–155
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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