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Direct attachment of DNA to semiconducting surfaces for biosensor applications

Paper ID Volume ID Publish Year Pages File Format Full-Text
23972 43486 2010 3 PDF Available
Title
Direct attachment of DNA to semiconducting surfaces for biosensor applications
Abstract

In this work we propose a novel method of immobilizing nucleic acids for field effect or high electron mobility transistor-based biosensors. The naturally occurring 5′ terminal phosphate group on nucleic acids was used to coordinate with semiconductor and metal oxide surfaces. We demonstrate that DNA can be directly immobilized onto ZrO2, AlGaN, GaN, and HfO2 while retaining its ability to hybridize to target sequences with high specificity. By directly immobilizing the probe molecule to the sensor surface, as opposed to conventional crosslinking strategies, the number of steps in device fabrication is reduced. Furthermore, hybridization to target strands occurs closer to the sensor surface, which has the potential to increase device sensitivity by reducing the impact of the Debye screening length.

Keywords
Nucleic acid; Detection; Immobilization; Semiconductor; Electronic; DNA
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Direct attachment of DNA to semiconducting surfaces for biosensor applications
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 150, Issue 3, November 2010, Pages 312–314
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us