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High level production of bioactive di- and tri-tyrosine peptides by protease-catalyzed reactions

Paper ID Volume ID Publish Year Pages File Format Full-Text
23976 43486 2010 5 PDF Available
Title
High level production of bioactive di- and tri-tyrosine peptides by protease-catalyzed reactions
Abstract

Papain-catalyzed polymerization of l-tyrosine ethyl ester in aqueous media was efficient for synthesis of oligo-tyrosine peptides having angiotensin I-converting enzyme inhibitory activity. Di-, tri-, and tetra-tyrosine accumulated in the soluble fraction of reaction mixture. On the other hand, the peptide products with degree of polymerization from 5 to 10 were insoluble, yields of which were influenced by initial concentrations of the ester substrate. The precipitated products could be used as substrates for α-chymotrypsin in DMSO/buffer systems producing soluble oligo-tyrosine peptides. In the reaction media containing DMSO at 40–50% (v/v), most of the precipitates were converted to soluble peptides. The two-step enzymatic reaction, that is papain-catalyzed synthesis of Tyr-polymers from l-tyrosine ethyl ester followed by their hydrolytic cleavage by α-chymotrypsin, is expected to be a potent procedure for synthesis of biologically active di- and tri-tyrosine peptides in good yield.

Keywords
ACE, angiotensin I-converting enzyme; ATEE, N-acetyl-l-tyrosine ethyl ester; DP, degree of polymerization; MALDI-TOFMS, matrix-assisted laser desorption/ionization time of flight mass spectrometry; RP-HPLC, reverse-phase high-performance liquid chromatogr
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High level production of bioactive di- and tri-tyrosine peptides by protease-catalyzed reactions
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 150, Issue 3, November 2010, Pages 343–347
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us