fulltext.study @t Gmail

DNA biosensor for detection of Neisseria gonorrhoeae causing sexually transmitted disease

Paper ID Volume ID Publish Year Pages File Format Full-Text
23978 43486 2010 9 PDF Available
Title
DNA biosensor for detection of Neisseria gonorrhoeae causing sexually transmitted disease
Abstract

A sequence-specific electrochemical sexually transmitted disease (STD) sensor based on self-assembled monolayer of thiolated DNA probe specific to target opa gene for detection of Gonorrhoea, a sexually transmitted disease has been fabricated. 6-Mercapto-1-hexanol (MCH) has been used as a blocking agent to facilitate oligos “stand” up at the surface, a configuration favoring subsequent DNA hybridization and to repel non-specific adsorption of undesired DNA. The results of differential pulse voltammetric studies of this STD sensor reveal low detection limit (1.0 × 10−18 M) and a wide dynamic range (from 1.0 × 10−6 M to 0.5 × 10−18 M) arising due to the stable hybridization using methylene blue as an electro-active DNA hybridization indicator. The experimental results with genomic DNA, clinical patient sample of Neisseria gonorrhoeae, culture of non-N. gonorrhoeae Neisseria species (NgNS) and gram negative bacteria indicate that the fabricated sensor is specific to this STD.

Keywords
Sexually transmitted disease; Neisseria gonorrhoeae; DNA biosensor; Gold electrode; Methylene blue; Electrochemical sensor
First Page Preview
DNA biosensor for detection of Neisseria gonorrhoeae causing sexually transmitted disease
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 150, Issue 3, November 2010, Pages 357–365
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us