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Integrated set-up of sampling, sample treatment and assay of an intracellular protein during fermentation

Paper ID Volume ID Publish Year Pages File Format Full-Text
23979 43486 2010 6 PDF Available
Title
Integrated set-up of sampling, sample treatment and assay of an intracellular protein during fermentation
Abstract

A complete system for on-line sampling, sample handling and analysis of fermentation processes has been set up. The system is designed such that a sample is taken from the fermentor, the metabolism of microbial cells is instantaneously arrested, the cells are lysed in order to release intracellular compounds and in a subsequent binding assay the content of target molecules is monitored. The system is constructed based on the flow-injection analysis concept. Good agreement between on-line analysis and off-line analysis is reported.

Keywords
On-line assay; FIA; PAT; Sampling; Intracellular protein
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Integrated set-up of sampling, sample treatment and assay of an intracellular protein during fermentation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 150, Issue 3, November 2010, Pages 366–371
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us