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Modulating and modeling aggregation of cell-seeded microcarriers in stirred culture system for macrotissue engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
23988 43486 2010 9 PDF Available
Title
Modulating and modeling aggregation of cell-seeded microcarriers in stirred culture system for macrotissue engineering
Abstract

A recently developed protocol, “microtissue assembly” holds great promise to address the issue of limited mass transfer within engineered large tissue replacements (macrotissues), wherein small “building blocks” (microtissues) are prepared and then assembled into macrotissues. Previous studies suggested that aggregation behavior of microcarrier-based microtissues were very important for macrotissue engineering. However, a systematic study on the aggregation behavior of microtissues is still missing. In this study, to examine the aggregation behavior of microtissues, effects of key operation parameters in dynamic culture including cell seeding density, microcarrier concentration, l-ascorbic acid 2-phosphate (Vc) and agitating speed were investigated. The aggregation process could be divided into three phases (i.e., lag, growth and stable). Aggregation efficiency (S) was found to be modulated by cell seeding density, microcarrier concentration, addition of Vc and agitating speed. A mathematical model correlating the operation parameters with S at different phases of aggregation was developed and experimentally proved to be able to predict S with varied operation parameters. In the end, a cylindrical macrotissue (diameter × height: 2.0 cm × 0.8 cm) with fairly good integrity and cellularity and uniform cell distribution was successfully engineered through perfusion assembling microtissues with controlled S under selected culture conditions. Our study showed that aggregation of microtissues could be precisely modulated, which would definitely facilitate engineering macrotissues with high quality.

Keywords
Microcarriers; Macrotissue engineering; Aggregation behavior; Bioreactor
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Modulating and modeling aggregation of cell-seeded microcarriers in stirred culture system for macrotissue engineering
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 150, Issue 3, November 2010, Pages 438–446
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us