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Construction of a thermostable cell adhesion protein for reverse transfection

Paper ID Volume ID Publish Year Pages File Format Full-Text
23989 43486 2010 5 PDF Available
Title
Construction of a thermostable cell adhesion protein for reverse transfection
Abstract

Transfection arrays are useful to analyze multiple genes at one time. In order to carry out gene transfection, cells are cultured on a plate on which genes are spotted to make extracellular matrix. However, this method is limited by low cell adhesion and transfection efficiency. To overcome these problems, we attempted to construct a novel extracellular matrix protein consisting of a variety of functional peptides. Here we fused the elastin derived peptide Ala-Pro-Gly-Val-Gly-Val (APGVGV) with the cell adhesive peptides, Pro-His-Ser-Arg-Asn (PHSRN) and Arg-Gly-Asp (RGD). The resulting fusion proteins, E12PSGR, had high cell adhesive activity, transfection efficiency, and thermal stability.

Keywords
ECM (extracellular matrix); Cell adhesion; RGD peptide; Thermal stability; Gene transfection
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Construction of a thermostable cell adhesion protein for reverse transfection
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 150, Issue 3, November 2010, Pages 447–451
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us