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Evidencing the role of lactose permease in IPTG uptake by Escherichia coli in fed-batch high cell density cultures

Paper ID Volume ID Publish Year Pages File Format Full-Text
23995 43488 2012 8 PDF Available
Title
Evidencing the role of lactose permease in IPTG uptake by Escherichia coli in fed-batch high cell density cultures
Abstract

The lac-operon and its components have been studied for decades and it is widely used as one of the common systems for recombinant protein production in Escherichia coli. However, the role of the lactose permease, encoded by the lacY gene, when using the gratuitous inducer IPTG for the overexpression of heterologous proteins, is still a matter of discussion. A lactose permease deficient strain was successfully constructed. Growing profiles and acetate production were compared with its parent strain at shake flask scale. Our results show that the lac-permease deficient strain grows slower than the parent in defined medium at shake flask scale, probably due to a downregulation of the phosphotransferase system (PTS). The distributions of IPTG in the medium and inside the cells, as well as recombinant protein production were measured by HPLC–MS and compared in substrate limiting fed-batch fermentations at different inducer concentrations. For the mutant strain, IPTG concentration in the medium depletes slower, reaching at the end of the culture higher concentration values compared with the parent strain. Final intracellular and medium concentrations of IPTG were similar for the mutant strain, while higher intracellular concentrations than in medium were found for the parent strain.Comparison of the distribution profiles of IPTG of both strains in fed-batch fermentations showed that lac-permease is crucially involved in IPTG uptake. In the absence of the transporter, apparently IPTG only diffuses, while in the presence of lac-permease, the inducer accumulates in the cytoplasm at higher rates emphasizing the significant contribution of the permease-mediated transport.

► IPTG uptake by Escherichia coli is a controversial issue. ► No direct IPTG measurements were available to date. ► We measured IPTG in E. coli high-cell density cultures. ► Lac-permease mediated transport might occur in E. coli LacY+. ► IPTG concentration in medium and intracellular is comparable in LacY−.

Keywords
Lac-permease; LacY gene; Recombinant protein production; Fed-batch fermentation; IPTG uptake
First Page Preview
Evidencing the role of lactose permease in IPTG uptake by Escherichia coli in fed-batch high cell density cultures
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 157, Issue 3, 10 February 2012, Pages 391–398
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering