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α-Chymotrypsin-catalyzed synthesis of poly-l-cysteine in a frozen aqueous solution

Paper ID Volume ID Publish Year Pages File Format Full-Text
24001 43488 2012 9 PDF Available
Title
α-Chymotrypsin-catalyzed synthesis of poly-l-cysteine in a frozen aqueous solution
Abstract

Poly-l-cysteine (PLCys) is drawing attention as a potential sorbent of thiol (SH)-reactive toxic heavy metal ions in the wastewater and polluted soils. However, preparation of PLCys relies on chemically synthesized polymers, in which SH groups must be protected and deprotected prior to use. On the other hand, α-chymotrypsin polymerized l-cysteine ethyl ester in a frozen aqueous solution, provides PLCys with degree of polymerization from 6 to 11 without blocking of SH groups. Kinetic analyses suggested that the acylation of α-chymotrypsin with the initial substrate was a rate-limiting step in the enzymatic polymerization. The peptide yields reached 85% and 65% of SH groups in PLCys were assumed to be free forms. Although detail information on correlation between the state of SH groups and heavy metal adsorption properties of PLCys should be explored in further studies, the present study for the first time proposed an easy method for synthesis of PLCys requiring neither SH-protection nor -deprotection.

Keywords
ATEE, N-acetyl-l-tyrosine ethyl ester; Cys-OEt, l-cysteine ethyl ester; DP, degree of polymerization; DTNB, 5,5′-dithiobis (2-nitrobenoic acid); MALDI-TOFMS, matrix assisted laser desorption/ionization time of flight mass spectrometry; PLCys, poly-l-cyste
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α-Chymotrypsin-catalyzed synthesis of poly-l-cysteine in a frozen aqueous solution
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 157, Issue 3, 10 February 2012, Pages 428–436
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Online Support
Any Questions? feel free to contact us