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Limited beneficial effects of perfluorocarbon emulsions on encapsulated cells in culture: Experimental and modeling studies

Paper ID Volume ID Publish Year Pages File Format Full-Text
24007 43489 2010 8 PDF Available
Title
Limited beneficial effects of perfluorocarbon emulsions on encapsulated cells in culture: Experimental and modeling studies
Abstract

Due to the high solubility of oxygen in perfluorocarbons (PFCs), these compounds have been explored for improved cell and tissue oxygenation. The goal of this study is to investigate the effects of a PFC emulsion on cellular growth and function in a tissue engineered construct. A perfluorotributylamine (PFTBA) emulsion was co-encapsulated at 10 vol% with mouse βTC-tet insulinoma cells in calcium alginate beads and cultured under normoxic and severely hypoxic conditions. The number of metabolically active cells and the induced insulin secretion rate were measured over time for up to 16 days. Results showed no significant effect of PFTBA relative to the PFTBA-free control. The alginate–PFC-cell system was also modeled mathematically, and simulations tracked the number of viable cells over time under the same conditions used experimentally. Simulations revealed only a small, likely experimentally undetectable difference in cell density between the PFC-containing and PFC-free control beads. It is concluded that PFTBA up to 10 vol% has no significant effect on the growth and function of encapsulated βTC-tet cells under normoxic and hypoxic conditions.

Keywords
AIDO, average intrabead dissolved oxygen; APA, alginate/PLL/alginate; DMEM, Dulbecco's Modified Eagle's Medium; DO, dissolved oxygen; GCR, glucose consumption rate; ISR, insulin secretion rate; PFC, perfluorocarbon; PFTBA, perfluorotributylamine; PLL, pol
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Limited beneficial effects of perfluorocarbon emulsions on encapsulated cells in culture: Experimental and modeling studies
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 150, Issue 2, 15 October 2010, Pages 232–239
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us