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Establishment of an Atlantic salmon kidney cell line with an inducible gene expression system

Paper ID Volume ID Publish Year Pages File Format Full-Text
24016 43490 2011 3 PDF Available
Title
Establishment of an Atlantic salmon kidney cell line with an inducible gene expression system
Abstract

A stable recombinant Atlantic Salmon Kidney cell line ASK for use as an inducible expression system was isolated, cloned and characterised. The cells were transfected with the pTet-Off plasmid from the Tet On/Off Clontech system, carrying a G418 resistance gene. Several G418-resistant clones were sub-cultured and characterised by qPCR and by transient transfection. The level of expression of transcriptional activator (tTA) was measured by qPCR in a number of isolated clones. Transient transfection with a pTRE2-hyg-LUC plasmid was used to evaluate the inducibility of these clones. Two clones were chosen for their compromise between cell growth and inducibility. This genetically engineered cell line is a valuable tool for the fish research community especially in research areas investigating the biological function of viral proteins.

► The first Atlantic salmon permanent inducible expression cell line has been isolated. ► Expression of the gene of luciferase was dependent on tetracyclin derivative in ASK-TOF. ► Valuable system to characterise gene biological functions in salmonids.

Keywords
Tet-off; ASK; Atlantic salmon; recombinant; Stable transfection
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Establishment of an Atlantic salmon kidney cell line with an inducible gene expression system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 154, Issue 4, 20 July 2011, Pages 209–211
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us