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Efficient disulfide bond formation in virus-like particles

Paper ID Volume ID Publish Year Pages File Format Full-Text
24020 43490 2011 10 PDF Available
Title
Efficient disulfide bond formation in virus-like particles
Abstract

Virus-like particles (VLPs) consist of a virus's outer shell but without the genome. Similar to the virus, VLPs are monodisperse nano-capsules which have a known morphology, maintain a high degree of symmetry, and can be engineered to encapsidate the desired cargo. VLPs are of great interest for vaccination, drug/gene delivery, imaging, sensing, and material science applications. Here we demonstrate the ability to control the disulfide bond formation in VLPs by directly controlling the redox potential during or after production and assembly of VLPs. The open cell-free protein synthesis environment, which has been reported to produce VLPs at yields comparable or greater than traditional in vivo technologies, was employed. Optimal conditions for disulfide bond formation were found to be VLP dependent, and a cooperative effect in the formation of such bonds was observed.

Keywords
VLP, virus-like particle; CFPS, cell-free protein synthesis; IAM, iodoacetamide; HBc VLP, human Hepatitis B core virus-like particle; HBcAg, human Hepatitis B core antigen; GSSG, oxidized glutathione; GSH, reduced glutathione; SS-CFPS, cell-free protein s
First Page Preview
Efficient disulfide bond formation in virus-like particles
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 154, Issue 4, 20 July 2011, Pages 230–239
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering