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Foaming of proteins: New prospects for enzyme purification processes

Paper ID Volume ID Publish Year Pages File Format Full-Text
24037 43491 2011 7 PDF Available
Title
Foaming of proteins: New prospects for enzyme purification processes
Abstract

Efficient techniques for the isolation of enzymes from a microbial production culture are required to meet the growing needs of the “White Biotechnologies” for novel catalysts. Traditional protein purification procedures typically comprise multistep operations, which inevitably come along with significant losses of enzyme activity. Foaming offers an alternative minimizing the processing steps, preserving the purification efficiency and decreasing the activity losses all at the same time. This review provides an insight into the foaming process itself and its application in separating enzymes from model systems and from complex media, such as microbial cultures. Examples demonstrate fractionated foaming and the tweezer technique.

Keywords
Foam fractionation; Purification; Enzymes; Proteins
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Foaming of proteins: New prospects for enzyme purification processes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 152, Issue 4, 10 April 2011, Pages 125–131
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us