fulltext.study @t Gmail

A genetic system for RNase E variant-controlled overproduction of ColE1-type plasmid DNA

Paper ID Volume ID Publish Year Pages File Format Full-Text
24042 43491 2011 5 PDF Available
Title
A genetic system for RNase E variant-controlled overproduction of ColE1-type plasmid DNA
Abstract

Escherichia coli endoribonuclease RNase E (Rne) regulates replication of ColE1-type plasmids by cleaving RNA I transcripts, which are synthesized from the plasmid and regulate the plasmid replication as antisense RNA. Here, we report the development of a genetic system that efficiently overproduces ColE1-type plasmid DNA when an RNase E variant that confers a hyperactive phenotype in RNA I cleavage is conditionally expressed from chromosome. This genetic system offers a method for isolation of large quantities of pure ColE1-type plasmid DNA, which have been most commonly used as molecular biology and biotechnology tools for research and industrial purposes.

Keywords
ColE1-type plasmid; Plasmid DNA; RNA I; RNase E; N-Rne
First Page Preview
A genetic system for RNase E variant-controlled overproduction of ColE1-type plasmid DNA
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 152, Issue 4, 10 April 2011, Pages 171–175
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us