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Analysis of adsorption of a baculovirus bioreaction bulk on an ion-exchange surface by surface plasmon resonance

Paper ID Volume ID Publish Year Pages File Format Full-Text
24048 43492 2010 11 PDF Available
Title
Analysis of adsorption of a baculovirus bioreaction bulk on an ion-exchange surface by surface plasmon resonance
Abstract

The binding and elution of the key components of a bioreaction bulk for the production of recombinant baculoviruses—a promising viral vector for gene therapy and vaccination—on a model ion-exchange surface have been successfully measured and interpreted by surface plasmon resonance (SPR) spectroscopy. The micro-scaled, ion-exchange surface was produced by immobilizing a typical ion-exchange ligand, diethylaminoethyl, onto commercially available planar gold sensor chip surfaces, which were pre-derivatized with a self-assembled monolayer of 11-mercaptoundecanoic acid. Each isolated analyte was injected into the SPR cell at defined operating conditions of salt and solute concentrations to determine the adsorption equilibrium plateau, and then eluted at the same salt concentration, upon which a well-defined, residual desorption equilibrium plateau was observed. From the analysis of the binding and elution curves and equilibrium plateaus for seven key biomolecules, it is possible to determine the adsorption isotherms over a broad range of equilibrium conditions for the three main cuts of the baculovirus bioreaction bulk: the product (the infective baculovirus), the main product-related impurities, and the main process-related impurities. A model that quantitatively explains the SPR-derived adsorption/desorption data was successfully developed for this complex biological system.

Keywords
Surface plasmon resonance; Ion-exchange chromatography; Scaled-down model for process development; Predictive modeling; Baculovirus; Biopharmaceutical
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Analysis of adsorption of a baculovirus bioreaction bulk on an ion-exchange surface by surface plasmon resonance
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 148, Issue 4, 2 August 2010, Pages 171–181
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us