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Immobilization of laccase in κ-carrageenan based semi-interpenetrating polymer networks

Paper ID Volume ID Publish Year Pages File Format Full-Text
24053 43492 2010 5 PDF Available
Title
Immobilization of laccase in κ-carrageenan based semi-interpenetrating polymer networks
Abstract

Laccase enzyme (L) was immobilized by entrapment into semi-interpenetrating polymer networks (semi-IPNs) prepared from κ-carrageenan with either poly(acrylamide–acrylic acid) [P(AAm–AA)/κ-car] or poly(acrylamide–itaconic acid) [P(AAm–IA)/κ-car]. For both systems, immobilized enzymes achieved the same optimum values observed for free enzyme (pH = 5.0 and T = 40 °C), except for P(AAm–IA)/κ-car system there was a shift to 5.5 in optimum pH value. At the end of 42 days of storage immobilized enzymes retained more than 80% of their original activities while the retained activities of both systems after 10 uses in batch type application were found to be higher than 50%. Km values were calculated as 2.52 × 10−2 mM and 1.08 × 10−2 mM and Vmax values were found as 6.8 × 10−3 mM min−1 and 4.4 × 10−3 mM min−1, for P(AAm–AA)/κ-car–L and P(AAm–IA)/κ-car–L, respectively. When methyl orange containing solutions (10 mg/L) were treated with the immobilized laccases (68.2 U), enzymatic decolorization of methyl orange in 6 h was achieved to the level of 35% for both systems. Supplementing the reaction medium with ABTS as the redox mediator increased this value to about 70%. These initial results show that, laccase containing semi-IPNs can find some applications in decolorization of the industrial wastes.

Keywords
Semi-IPNs; Immobilized laccase; κ-Carrageenan; Decolorization; Methyl orange
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 148, Issue 4, 2 August 2010, Pages 216–220
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us