fulltext.study @t Gmail

Enzymatic hydrolysis and ethanol production using xyloglucanase and Debaromyces hansenii from tamarind kernel powder: Galactoxyloglucan predominant hemicellulose

Paper ID Volume ID Publish Year Pages File Format Full-Text
24056 43492 2010 7 PDF Available
Title
Enzymatic hydrolysis and ethanol production using xyloglucanase and Debaromyces hansenii from tamarind kernel powder: Galactoxyloglucan predominant hemicellulose
Abstract

The hydrolysis and ethanol production from tamarind kernel powder (TKP), a rich source of galactoxyloglucan (GXG) was investigated for the first time using xyloglucanase and thermotolerant Debaromyces hansenii. The acid hydrolysis of TKP with 2N H2SO4 at 120 °C for 30 min yielded an overall saccharification of 94% based on the total available carbohydrate content and further fermentation at 40 °C with thermotolerant D hansenii produced an ethanol yield of 0.35 g/g. A maximum hydrolysis of 55 and 78% for GXG was obtained in 48 h at 50 °C using Thermomonospora xyloglucanase (TXy) and accellerase™1000, respectively. The synergistic effect of β-galactosidase and xyloglucanase was demonstrated by the exogenous addition of β-galactosidase to TXy which improved the overall hydrolysis of GXG by 30%. The rate of hydrolysis of GXG with TXy and accellerase was increased by 15–20% in the presence of chemical surfactants (tween 80 and toluene) or protein additive (BSA). The fermentation of enzymatic hydrolysates of GXG by TXy and accellerase with free cells at 40 °C produced an ethanol yield of 0.39 and 0.41 g/g whereas with immobilized cells produced 0.45 and 0.43 g/g, respectively, with a theoretical conversion efficiencies of 78–88%. The immobilized yeast cells were reused six times at 40 °C with 100% fermentation efficiency.

Keywords
Tamarind kernel powder; Galactoxyloglucan; Xyloglucanase; Hydrolysis; Thermotolerant yeast; Ethanol
First Page Preview
Enzymatic hydrolysis and ethanol production using xyloglucanase and Debaromyces hansenii from tamarind kernel powder: Galactoxyloglucan predominant hemicellulose
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 148, Issue 4, 2 August 2010, Pages 233–239
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us