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Effective delivery of anti-miRNA DNA oligonucleotides by functionalized gold nanoparticles

Paper ID Volume ID Publish Year Pages File Format Full-Text
24062 43493 2011 6 PDF Available
Title
Effective delivery of anti-miRNA DNA oligonucleotides by functionalized gold nanoparticles
Abstract

MicroRNAs (miRNAs) are gaining recognition as essential regulators involved in many biological processes, and they are emerging as therapeutic targets for treating disease. Here, we introduce a method for effective delivery of anti-miRNA oligonucleotides (AMOs) using functionalized gold nanoparticles (AuNPs). To demonstrate the ability of AMOs to silence miRNA, we selected miR-29b, which is known to downregulate myeloid cell leukemia-1 (MCL-1), a factor responsible for promoting cell survival. We first generated AuNPs coated with cargo DNA, which was then coupled to complementary DNA linked to an antisense miR-29b sequence. When the AuNPs were delivered into HeLa cells, MCL-1 protein and mRNA levels were increased significantly. Furthermore, apoptosis induced by tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) was inhibited, proving that AMOs targeting miR-29b were effectively delivered by our innovative AuNP. In addition, we provided evidence that AuNP could deliver other AMOs against miR-21 into two independent cell lines, KGN and 293T, suggesting that the AuNP conjugates can be versatile for any AMO and cell type.

► Design of anti-miRNA oligonucleotides (AMOs) targeting miR-29b. ► Effective delivery of AMOs by functionalized gold nanoparticles (AuNP). ► AuNP-conjugated AMO-miR29b upregulates MCL-1 by blocking miR-29b. ► AuNP-conjugated AMO-miR29b protects against apoptotic stimulation with TRAIL.

Keywords
Anti-microRNA oligonucleotides (AMOs); Gold nanoparticles; miR-29; MCL-1
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Effective delivery of anti-miRNA DNA oligonucleotides by functionalized gold nanoparticles
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 155, Issue 3, 20 September 2011, Pages 287–292
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us