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An RNA–DNA hybridization assay chip with electrokinetically controlled oil droplet valves for sequential microfluidic operations

Paper ID Volume ID Publish Year Pages File Format Full-Text
24068 43493 2011 8 PDF Available
Title
An RNA–DNA hybridization assay chip with electrokinetically controlled oil droplet valves for sequential microfluidic operations
Abstract

A novel RNA–DNA hybridization microfluidic chip for detecting pathogens was developed. The on-chip sequential operations of reagent delivery and washing processes in the hybridization assay were performed by gravity-based pressure-driven flow controlled by a pair of electrokinetically controlled oil-droplet sequence valves (ECODSVs). Numerical method was used to simulate the fluidic processes of reagents in the complex microchannel network. Based on the parameters determined from the numerical simulations, a reasonable hybridization assay microfluidic chip was developed. The application of this on-chip assay to detect Salmonella was demonstrated. Significantly shortened assay time (25 min) and a 3–20-fold reduction in reagent/sample consumption were achieved. The detection limit was 103 CFU/mL which is comparable to the conventional assay. With further development of automatic control and the improvement of the detection strategy, this microfluidic RNA–DNA hybridization assay technique has a potential for point-of-testing applications.

Keywords
RNA–DNA hybridization; Microfluidic; Lab-on-chip; Electrokinetic; Salmonella
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An RNA–DNA hybridization assay chip with electrokinetically controlled oil droplet valves for sequential microfluidic operations
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 155, Issue 3, 20 September 2011, Pages 330–337
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us