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Coherent diffractive imaging of biological samples at synchrotron and free electron laser facilities

Paper ID Volume ID Publish Year Pages File Format Full-Text
24073 43494 2010 9 PDF Available
Title
Coherent diffractive imaging of biological samples at synchrotron and free electron laser facilities
Abstract

Coherent X-ray diffractive imaging (CXDI) is a new imaging technique that offers the potential to image non-crystalline materials to sub-nanometer resolutions. Here we review the progress in CXDI of biological samples at both synchrotron and free electron laser (FEL) sources. We outline the experimental design of a CXDI experiment and summarize the iterative phase retrieval techniques that are used to produce images from the measured diffraction patterns. We describe a selection of key experiments performed in bio-imaging with CXDI from synchrotron sources, and we discuss the proof-of-principle experiments performed at FLASH at DESY in Hamburg. Finally, we show through simulation that for realistic parameters of hard X-ray FELs a resolution of a few nanometers may be achieved for individual biological objects imaged with single pulses of FEL radiation. Furthermore, we revise how this resolution may be improved to the sub-nanometer range if we image multiple copies of samples with a reproducible structure.

Keywords
Coherent imaging; Phase retrieval; Biological imaging; Synchrotron; Free electron laser
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Coherent diffractive imaging of biological samples at synchrotron and free electron laser facilities
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 149, Issue 4, 15 September 2010, Pages 229–237
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us