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Soft X-ray holographic microscopy of chromosomes with high aspect ratio pinholes

Paper ID Volume ID Publish Year Pages File Format Full-Text
24074 43494 2010 5 PDF Available
Title
Soft X-ray holographic microscopy of chromosomes with high aspect ratio pinholes
Abstract

We used digital in-line soft X-ray holography (DIXH) in the Gabor geometry to image human chromosomes. The divergent wave front was generated by diffraction of synchrotron radiation from a high aspect ratio pinhole. As under our experimental conditions the achievable resolution depends on the pinhole radius, high aspect ratio holes with diameters in the 100 nm range were prepared by focused ion beam (FIB) milling. The central maximum of the obtained Airy pattern was used to image chromosomes prepared from metaphase HeLa cells at experimental resolutions of 370 ± 40 nm with soft X-rays at 260 eV photon energy provided by the BESSY II synchrotron radiation facility.

Keywords
Digital holography; Image analysis; X-ray imaging; Synchrotron radiation; X-ray microscopy; Soft X-rays
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 149, Issue 4, 15 September 2010, Pages 238–242
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us