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Fluorescence microscopy beyond the diffraction limit

Paper ID Volume ID Publish Year Pages File Format Full-Text
24075 43494 2010 9 PDF Available
Title
Fluorescence microscopy beyond the diffraction limit
Abstract

In the recent past, a variety of fluorescence microscopy methods emerged that proved to bypass a fundamental limit in light microscopy, the diffraction barrier. Among diverse methods that provide subdiffraction spatial resolution, far-field microscopic techniques are in particular important as they can be operated in complex biological samples such as cells or tissue. Valuable new insights into biomolecular structure, organization and even dynamic processes in living cells have been gained with these novel microscopic techniques. In the present review, the most important concepts of far-field microscopy with subdiffraction resolution are introduced. The underlying physical concepts are discussed, and practical considerations for the application of these methods are made.

Keywords
Fluorescence microscopy; Subdiffraction resolution; Super-resolution; Stimulated-emission depletion; Structured-illumination microscopy; Photoswitching microscopy
First Page Preview
Fluorescence microscopy beyond the diffraction limit
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 149, Issue 4, 15 September 2010, Pages 243–251
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering