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On the origin of cell functions encoded in the toponome

Paper ID Volume ID Publish Year Pages File Format Full-Text
24076 43494 2010 8 PDF Available
Title
On the origin of cell functions encoded in the toponome
Abstract

The fluorescence imaging technology TIS enables the investigator to locate and decipher functional protein networks (the toponome) in a single cell or tissue section. TIS permits optical resolution and simultaneous detection of thousands of protein clusters in situ, composed of different protein species, and their visualization as coloured mosaic structures. Access to this level of protein organization relies on the ability of TIS to break the spectral limit of fluorescence microscopy and co-map a quasi unlimited number of different proteins by using specific tag libraries. The present review outlines the principles of the TIS technology as a fundamental approach to the internal structure, the code and the semantics of any protein system in situ. The review focusses on the discovery of basic coding rules in the toponome, indicating that cells establish functional protein networks on the cell surface by interlocking protein clusters, in which highly dissimilar proteins are topologically assembled (dissimilarity rule), and each cluster exhibits a characteristic geometry on the submicrometer to micrometer scale (geometry rule). The network is hierarchically controlled by a lead protein, whose inhibition leads to disassembly of the network and loss of function. Use of TIS on a proteome-wide scale provides a new way to medical systems biology.

Keywords
Toponome; Proteome; TIS; MELC; Protein networks; Systems biology; Cell surface
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On the origin of cell functions encoded in the toponome
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 149, Issue 4, 15 September 2010, Pages 252–259
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us