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Automated detection and quantification of fluorescently labeled synapses in murine brain tissue sections for high throughput applications

Paper ID Volume ID Publish Year Pages File Format Full-Text
24082 43494 2010 11 PDF Available
Title
Automated detection and quantification of fluorescently labeled synapses in murine brain tissue sections for high throughput applications
Abstract

The automated detection and quantification of fluorescently labeled synapses in the brain is a fundamental challenge in neurobiology. Here we have applied a framework, based on machine learning, to detect and quantify synapses in murine hippocampus tissue sections, fluorescently labeled for synaptophysin using a direct and indirect labeling method with FITC as fluorescent dye. In a pixel-wise application of the classifier, small neighborhoods around the image pixels are mapped to confidence values. Synapse positions are computed from these confidence values by evaluating the local confidence profiles and comparing the values with a chosen minimum confidence value, the so called confidence threshold. To avoid time-consuming hand-tuning of the confidence threshold we describe a protocol for deriving the threshold from a small set of images, in which an expert has marked punctuate synaptic fluorescence signals. We can show that it works with high accuracy for fully automated synapse detection in new sample images. The resulting patch-by-patch synapse screening system, referred to as i3S (intelligent synapse screening system), is able to detect several thousand synapses in an area of 768 × 512 pixels in approx. 20 s. The software approach presented in this study provides a reliable basis for high throughput quantification of synapses in neural tissue.

Keywords
Bioimage analysis; Neural tissue; Fluorescence microscopy; Synaptophysin staining; Support vector machine; High throughput synapse detection
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Automated detection and quantification of fluorescently labeled synapses in murine brain tissue sections for high throughput applications
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 149, Issue 4, 15 September 2010, Pages 299–309
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us