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Sorbitol production using recombinant Zymomonas mobilis strain

Paper ID Volume ID Publish Year Pages File Format Full-Text
24087 43496 2010 8 PDF Available
Title
Sorbitol production using recombinant Zymomonas mobilis strain
Abstract

A recombinant Zymomonas mobilis strain harboring the plasmid pHW20a-gfo for over-expression of glucose–fructose oxidoreductase (GFOR) was constructed. The specific activity of GFOR enzyme in the new recombinant strain was at least two folds greater than that in the wild strain. The maximum GFOR activity achieved in terms of the volumetric, and the cellular were 2.59 U ml−1, and 0.70 U mg−1, respectively, in the batch cultures. A significant improvement of the bioconversion process for the production of sorbitol and gluconic acid from glucose and fructose was made using divalent metal ions which drastically reduced the ethanol yield and significantly increased the yield of target product. Among several divalent metal ions evaluated, Zn2+ was found to be most effective by inhibiting the Entner–Doudoroff pathway enzymes. The yield of the byproduct ethanol was reduced from 16.7 to 1.8 g l−1 and the sorbitol yield was increased to almost 100% from 89%. The Ca2+ enhanced the sorbitol yield and the formation of calcium gluconate salt made the separation of gluconate from the reaction system easier.

Keywords
Recombinant Zymomonas mobilis; Glucose–fructose oxidoreductase (GFOR); Sorbitol; Permeabilization free; Metal ion addition; Inhibitors
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Sorbitol production using recombinant Zymomonas mobilis strain
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 148, Issues 2–3, 20 July 2010, Pages 105–112
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us