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Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
24090 43496 2010 5 PDF Available
Title
Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells
Abstract

Valproic acid (VPA) is a small molecule that inhibits histone deacetylase activity. Here we report that VPA increases recombinant mRNA and protein levels in transiently transfected CHO DG44 cells. In the presence of VPA, transient recombinant antibody yields of up to 40 mg/L were achieved in simple batch cultures. The steady-state levels of the IgG light and heavy chain mRNAs were nearly 10 times higher than in the untreated control transfection even though the level of transfected plasmid DNA was the same in the presence or absence of VPA. The combination of VPA treatment and incubation of the transfected cells in mildly hypothermic conditions resulted in recombinant antibody yields of over 90 mg/L by 6 days post-transfection in batch cultures. The results demonstrated that the treatment of transfected CHO DG44 cells with VPA is a cost-effective strategy for enhancing transient gene expression by increasing the transgene mRNA levels.

Keywords
VPA, valproic acid; VPD, valpromide; PEI, polyethylenimine; RhD, Rhesus D antigen; GFP, green fluorescent protein; HDAC, histone deacetylase; iHDAC, histone deacetylase inhibitor; TGE, transient gene expression; CHO, Chinese hamster ovary; HEK-293, human
First Page Preview
Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 148, Issues 2–3, 20 July 2010, Pages 128–132
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering