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Genome shuffling of Propionibacterium shermanii for improving vitamin B12 production and comparative proteome analysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
24092 43496 2010 5 PDF Available
Title
Genome shuffling of Propionibacterium shermanii for improving vitamin B12 production and comparative proteome analysis
Abstract

Genome shuffling is an efficient approach for the rapid improvement of microbial phenotype. Here we improved vitamin B12 production of Propionibacterium shermanii by genome shuffling based on inactivated protoplast fusion. A genome shuffling strain with titer of vitamin B12 of 2.85 mg l−1, named Propionibacterium shermanii-F2-3, was obtained. The genome shuffled strain produced about 61% improvement of vitamin B12 over the parent strain after 96 h. Comparative analysis of proteome profile was conducted between Propionibacterium shermanii 17 and F2-3. The expression levels of 38 proteins varied significantly in the genome shuffled strain compared with those in the parent strain. Of these proteins, 22 proteins were up-regulated, 16 proteins were down-regulated. Of the up-regulated proteins, 6 proteins (glutaminyl-tRNA synthetase (GlnS), Delta-aminolevulinic acid dehydratase (HemB), methionine synthase (Meth), riboflavin synthase (RibE), phosphofructo kinase (PfkA) and isocitrate dehydrogenase (Icd) is involved in the vitamin B12 biosynthesis pathway. They may be the key enzymes of vitamin B12 biosynthesis.

Keywords
Vitamin B12; Genome shuffling; Propionibacterium shermanii; Proteome analysis
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Genome shuffling of Propionibacterium shermanii for improving vitamin B12 production and comparative proteome analysis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 148, Issues 2–3, 20 July 2010, Pages 139–143
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us